Impact of irradiation dose on tumor cells"proliferation and tumor fusion vaccines"effection
10.3969/j.issn.1006-5725.2018.04.003
- VernacularTitle:辐照剂量对肿瘤细胞活性及肿瘤融合细胞疫苗效果的影响
- Author:
Yating YU
1
;
Siliang DUAN
;
Xinbo MA
;
Haiguang SHEN
;
Yun LIU
;
Bojin JIANG
;
Sheng ZHOU
;
Libing XIAO
;
Lihua WEI
Author Information
1. 广西科技大学医学院病原生物学与免疫学教研室
- Keywords:
fusion cells;
irradiation dose;
tumor;
vaccines
- From:
The Journal of Practical Medicine
2018;34(4):527-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective An optimal radiation dose was made research on tumor cells to develop effective tumor fusion vaccines. Methods We used the RS 2000 biological X-ray radiation instrument to produce different dose of X-rays.Mouse liver cancer cells line,BNL 1ME a.7R.1(MEAR),was used for experiment.The RS 2000 biological X-ray radiation was applied to create different tumor cell clones,which could help us to study the rela-tionship between irradiation dose and tumor cells′proliferation activity.Furthermore,the fusion cells′anti-tumor ef-fect was examined by flow cytometry. Results High-dose radiation would induce the lower proliferation of cancer cells than low-dose irradiation do.Conclusions During the preparation of fusion vaccines,irradiation dose should be considered as a factor that would influence the tumor cells′ proliferation activity. When the dose of irradiation was appropriate,we could make safe and efficient integration cell vaccines.
