Preparation of magnetic mNGF nanoparticles and treatment of rat sciatic nerve injury guided by outside magnetic field
10.3969/j.issn.1006-5725.2018.03.010
- VernacularTitle:磁性载mNGF纳米微粒的制备及外磁场引导下修复大鼠坐骨神经损伤的实验研究
- Author:
Lijun LI
1
;
Hamid
;
Qiang ZONG
;
Shuai WANG
;
Liang WANG
;
Dongkui NI
Author Information
1. 天津医科大学第二医院骨科 天津300211
- Keywords:
magnetic mNGF nanoparticle;
outside magnetic field;
sciatic nerve injury;
nerve regen-eration
- From:
The Journal of Practical Medicine
2018;34(3):381-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the experimental effectofmagnetic mNGF nanoparticlesguided by out-side magnetic on the the sciatic nerve injury.Tianjin 300211,China.Methods As a drug carrier,PLGA connects magneticironparticles with and mNGF. The nanoparticle suspension liquid was prepared and its physicochemical properties was characterized. The model of the left sciatic nerve injury in rats was divided into 3 groups. Rats in Group A received intravenous tail vein injectionof magnetic mNGF nanoparticlesfollowed by two-hour outside mag-neticguidance once week.Group B received intravenous tail vein injection of magnetic mNGF nanoparticles without outside magnetic guidance once week. Group C received intravenous tail vein injection of the same content mNGF once week. Two months later,we measured the ischial nerve index(SFI)and madenerve electrical physiological ex-amination under anesthesia.We also measured quality ratio of triceps surae and did left sciatic nerve slice HE stain-ing.Finally,experimental data were statistically analyzed. Results The SFI,nerve conduction velocity and quality ratio of triceps surae of Group A were higher than those of Group B and Group B(P<0.05).There was no significant difference between Group B and Group C(P>0.05).The arrangement and density of the regeneration nerve in Group A were better than those of Group B and C under optical microscope. Conclusion The results showed that the magnetic mNGF nanoparticles guided by outside magnetic can promote the recovery of rat sciatic nerve injury.
