Effects of propofol on proliferation and invasion of lung adenocarcinoma cell line by matrix metalloprotein-ases
10.3969/j.issn.1006-5725.2018.03.005
- VernacularTitle:异丙酚影响基质金属蛋白酶对肺腺癌细胞株增殖侵袭能力的作用
- Author:
Yongqiang ZHANG
1
;
Jun LIU
;
Shengyang CHEN
;
Guoze LIU
;
Jianmin TIAN
;
Xiuqin YUE
Author Information
1. 新乡医学院第一附属医院麻醉科 河南新乡453100
- Keywords:
propofol;
adenocarcinoma of the lung;
matrix metalloproteinase 2
- From:
The Journal of Practical Medicine
2018;34(3):357-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect and possible mechanism of propofol on proliferation and inva-sion of lung cancer cells. Methods Lung cancer cells were cultured and divided into experimental control group, groupL,group M and group H.The optical density of cancer cell was detected by CCK-8.Meanwhile,the difference of the proliferation of cancer cells in each groupwas determined after down-regulation gene.The change of inhibition rate in four groups was analyzed by Transwell test. The expression of MMP-2 and mRNA in different groups were compared by Western blot and Q-PCR. Results the results of CCK-8 test showed the cancer cell viability in H group was lowest and the inhibition ability increasedafter silencing MMP-2 gene(P<0.05); the invasion inhibition rate of the lung cancer cells treated with propofol was higher(P<0.05), and invasion inhibition rate in each group also rise after silencing MMP-2; Western blot and Q-PCR show that the MMP-2 expression level in group Mand group H were lowest(P<0.05).Conclusion Propofol may inhibit the proliferation and invasion of lung cancer cells and its mechanism may be associated with down-regulation MMP-2,which is expected to inhibit the proliferation of lung cancer invasion.
