Diagnostic value of detecting Mycobacterium tuberculosis rRNA via SAT in anti-TB treatment
10.3969/j.issn.1006-5725.2018.02.035
- VernacularTitle:SAT技术检测结核杆菌rRNA对肺结核的快速诊断价值
- Author:
Yunyi XU
1
;
Xingshan CAI
;
Yaoju TAN
;
Yanwen LIU
;
Shaofang ZENG
;
Pingyun MA
;
Huilin ZHOU
Author Information
1. 广州市胸科医院检验科 广州510095
- Keywords:
tuberculosis;
nucleic acid amplification technology;
diagnosis
- From:
The Journal of Practical Medicine
2018;34(2):297-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the clinical value of simultaneous amplification and testing for detection of Mycobacteria tuberculosis(SAT-TB)in sputum samples and bronchoalveolar lavage fluid(BALF)samples. Methods Totally 169 sputum samples and 151 BALF samples from suspected pulmonary tuberculosis patients were detected by both SAT and Bactec MGIT960.The sensitivity,specificity,positive predictive value(PPV)and negative predictive value(NPV)of the samples using SAT-TB were calculated. Results Taken the results of BD960 as the reference,the sensitivity,specificity,PPV and NPV using SAT-TB of sputum samples were 84.00% (42/50),93.06%(67/72),89.36%(42/47)and 89.33%(67/75)respectively;and those of BALF samples 89.19% (33/37),95.12%(39/41),94.29%(33/35)and 42.39%(39/92)respectively.Taken clinical diagnostic results as reference standard,the sensitivity,specificity,PPV and NPV using SAT-TB of the sputum samples were 57.73% (56/97),93.06%(67/72),91.80%(56/61),and 62.04%(67/108)respectively;and those of BALF samples 51.82%(57/110),94.29%(39/41),96.61%(57/59)and、42.39%(39/92)respectively.The sensitivity,specificity, PPV and NPV using BD960 of the sputum samples were 51.55%(50/97),95.83%(69/72),94.34%(50/53),and 59.48%(69/116)respectively;and those of BALF samples 33.64%(37/110),90.24%(37/41),90.24%(37/41) and 33.64%(37/110)respectively.Conclusion SAT-TB is a rapid and sensitive method for the detection of Myco-bacteria tuberculosis in sputum and BALF samples.It can improve the detection rate of mycobaterium tuberculosis.
