Effect of hydrogen on mitochondrial function after cerebral ischemia/reperfusion injury in rats
10.3969/j.issn.1006-5725.2018.02.005
- VernacularTitle:氢气对大鼠脑缺血/再灌注损伤线粒体功能的影响机制
- Author:
Xinlei ZHANG
1
;
Pan CHEN
;
Yuning XIA
;
Xuemei LI
;
Chunguang DAI
;
Yongxing TAN
Author Information
1. 桂林医学院研究生院 广西桂林541001
- Keywords:
cerebral ischemia reperfusion;
hydrogen;
ROS;
mitochondrial
- From:
The Journal of Practical Medicine
2018;34(2):184-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hydrogen on mitochondrial function in cerebral cortex after cerebral ischemia/reperfusion(I/R)injury in rats. Methods 48 male SD rats were randomly divided into sham operation group(sham group),brain I/R injury group(MOD group),hydrogen treatment group(H2group). 24 hours after reperfusion,the neurological deficit scoring was performed. The changes of mitochondrial membrane potential(△ψm),permeability openness(MPTP),ROS production rate and mitochondrial swelling were detected. Results Compared with the Sham group,neurological deficit score,MPTP openness,mitochondrial swelling degree and ROS production rate were increased in the MOD group(P<0.01),△ψm levels were reduced(P<0.01). Compared with the MOD group,the neurological deficit score,MPTP openness,mitochondrial swelling degree and ROS production rate were decreased in H2group(P<0.01),△ψm levels increased(P<0.01). Conclusions Simul-taneous intraperitoneal injection of pure hydrogen can reduce the generation of reactive oxygen species,protect the mitochondrial function of neuronal cells in the ischemic region after brain I/R,improvement the rat brain I/R after the neurological scoring.
