Role of RNA-binding protein Larp1 in ovarian cancer invasion and metastasis and mechanism
10.3969/j.issn.1006-5725.2018.01.006
- VernacularTitle:RNA结合蛋白Larp1在卵巢癌侵袭转移中的作用及机制
- Author:
Yan DAI
1
;
Shiguang JIN
;
Xuejie WANG
;
Xinquan YANG
;
Daxin WANG
Author Information
1. 江苏省苏北人民医院医学实验研究中心
- Keywords:
Larp1;
ovarian carcinoma;
EMT;
invasion and metastasis
- From:
The Journal of Practical Medicine
2018;34(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective Recent evidence has implicated Larp1,an RNA-binding protein,in cell motility and EMT therefore prompting the study of Larp1 in EOC.This project aims to examine the potential role of Larp1 in cell invasion.Methods Ovarian cancer cells SKOV-3 and OVCAR-3 were transfected with DNA constructs to overexpress Larp1 and transfect cells were used to assess the overexpression of Larp1 in cell invasion and metastasis using several functional assay.Results Larp1 overexpression in both OVCAR-3 and SKOV-3 cells appear to enhance the invasive ability of cells and the accumulation of Larp 1 in these chemoinvasive protrusions strongly suggests the potential role of Larp1 in invadopodia formation.Cell spot chemoinvasion assay demonstrated increased chemoinvasion of Larp1 overexpressing SKOV-3 and OVCAR-3 cells towards a gradient of TGF-β,EGF and bFGF.Conclusions With its potential role in EMT and cell invasion,a deeper understanding of the physiological role of Larp1 in cancer cell motility will be potentially beneficial in the diagnosis and treatments of ovarian cancer.
