Effect of dichloroacetate on colony-forming, invasion and migration of the T24 bladder cancer cells line and its mechanism
10.3969/j.issn.1006-5725.2018.01.005
- VernacularTitle:二氯乙酸对膀胱癌细胞株T24克隆形成、侵袭和迁移的影响
- Author:
Zhibin XIE
1
;
Weijin FU
;
Chunyu LU
;
Dong ZHAO
;
Yanzhen XU
;
Huayu WU
Author Information
1. 广西医科大学第一附属医院,南宁530021
- Keywords:
dichloroacetate;
bladder cancer;
colony-forming;
invasion;
migration;
epithelial-mesenchymal transition
- From:
The Journal of Practical Medicine
2018;34(1):16-20,25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of dichloroacetate (DCA) on cell colony-forming,cell invasion and cell migration of the bladder cancer cells and to study the underlying mechanism.Methods The bldder cancer cells T24 were randomly divided into two groups:the observation group and the control group.Cells in the observation groups were treated with 5 mmol/L,10 mmol/L and 20 mmol/L dichloroacetate,and the control group was treated with the same amount of dimethyl sulfoxide.Colony formation assays were detected with Giemsa staining.Cell wound scratch assay and Transwell assay were applied to evaluate the ability of the T24 cell invasion and migration.Realtime PCR and Western blot were applied to detect the expression of the epithelial-mesenchymal transition (EMT)-related marker,including E-cadherin,N-cadherin,vimentin,Snail and Slug.Results Compared with the control group,the colony formation assays of T24 cells constantly decreased along with the increased doses in the observation group(P ＜ 0.01).The cell wound scratch assay showed that the scratch width of the observation groups were significantly higher along with the increased doses and prolonged time than that in the control group (P ＜ 0.01).The transwell assay showed that the invasion ability of the observation groups were significantly discreased along with the increased doses than that in the the control group (P ＜ 0.01).The expression levels of E-cadherin mRNA and protein in combination the control group were higher than those in the the observation groups (P ＜ 0.05).However,the expression levels of N-cadherin,vimentin,Snail and Slug mRNAs and proteins in combination the control group were lower than those in the the observation groups (P ＜ 0.05).Conclusion Dichloroacetate can inhibit the colony-forming,invasion and migration of bladder cancer T24 cells,and its mechanism may inhibit the expression of epithelial mesenchymal transition in T24 cells by down-regulating the expression of nuclear transcription factor Snail and Slug.
