Relationship between intrapulmonaryTREM-1 and endoplasmic reticulum stress in mice with acute lung injury
10.3969/j.issn.1006-5725.2017.22.008
- VernacularTitle:急性肺损伤小鼠肺内TREM-1与内质网应激相关性研究
- Author:
Liang DONG
1
;
Dexing LIU
;
Yuhang ZHU
;
Yongqin CHEN
;
Zhaoqiong ZHU
Author Information
1. 563000,遵义医学院附属医院麻醉科
- Keywords:
acute lung injury;
triggering receptor expressed on myeloid cells-1;
endoplasmic reticulum stress;
macrophage
- From:
The Journal of Practical Medicine
2017;33(22):3710-3713
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the correlation of intrapulmonary TREM-1 with endoplasmic reticulum stress in mice with acute lung injury (ALI).Methods Balb/c mice were tracheally injected with lipopolysaccharide(LPS,5 mg/kg) to induce ALI.Real-time PCR and Western blot were used to detect the expressions of TREM-1,CHOP and GRP78.The correlation of TREM-1 with endoplasmic reticulum-related proteins was analyzed.LPS (100 ng/mL) was used to induce inflammation in mouse primary peritoneal macrophages,and expressions of TREM -1,CHOP and GRP78 mRNA were detected by real-time PCR.The effect of TREM-1 activation on the expressions of CHOP and GRP78 was observed in macrophages.Results The expressions of TREM-1,CHOP and GRP78 mRNA were increased in ALI mice.TREM-1 mRNA expression was positively correlated with CHOP and GRP78 mRNA expression.In vitro,LPS up-regulated the expressions of TREM-1,CHOP and GRP78,and TREM-1 was positively correlated with CHOP and GRP78.Activation of TREM-1 increased CHOP and GRP78 mRNA expressions.Conclusions TREM-1 is positively related to the endoplasmic reticulum stress.The activation of TREM-1 enhances endoplasmic reticulum in mouse macrophages.
