Alizarin induced the function of beta subunits of BKCa channel relaxed interlobar renal artery and reduced SHR systolic pressure
10.3969/j.issn.1006-5725.2017.22.007
- VernacularTitle:茜草素介导BKCa通道beta亚基功能舒张肾叶间动脉降低SHR收缩压
- Author:
Yanping ZHANG
1
;
Yuanyuan WANG
;
Yanjie GAN
;
Jianing WANG
;
Chuanglin ZHANG
;
Xiaowen LI
Author Information
1. 湖北医药学院附属人民医院心脏中心
- Keywords:
Alizarin;
interlobar renal artery;
BKCa channel;
smooth muscle cell;
whole cell patch clamp
- From:
The Journal of Practical Medicine
2017;33(22):3706-3709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of alizarin on BKCa channel beta subunit and electrophysiological characteristics in interlobar renal artery of SHR.Methods Tail artery pressure measurement instrument,pressure myograph system,whole cell patch clamp technique and Western blot were used to measure the change of systolic pressure of SHR,renal interlobar arterial diameter,single vascular smooth muscle cell electrophysiological characteristics and expression of the BKCa channel beta subunit before and after alizarin intervention,respectively.Results (1) SHR systolic pressure was decreased after alizarin intervention (P ＜ 0.01).(2) ibTX inhibited alizarin-mediated renal interlobar arterial relaxation (P ＜ 0.01).(3) Alizarin enhanced BKCa channel-mediated outward currents (P ＜ 0.05).(4) Alizarin up-regulated BKCa channel beta subunits after alizarin intervention (P ＜ 0.01).Conclusion Alizarin reduced SHR systolic pressure and relaxed interlobar renal artery by enhancement BKCa currents via mediation of the function of beta subunits.
