Tacrolimus inhibits podocyte injury and stabilizes the expression of cytoskeleton and nephrin
10.3969/j.issn.1006-5725.2017.22.004
- VernacularTitle:他克莫司通过稳定细胞骨架和nephrin抑制血管紧张素Ⅱ损伤肾小球足细胞
- Author:
Yueqiang WEN
1
;
Huiyuan LI
;
Peilan ZHOU
;
Zebin WANG
;
Jianbo LIANG
Author Information
1. 广州医科大学附属第二医院肾内科
- Keywords:
podocyte;
tacrolimus;
cytoskeleton;
nephrin
- From:
The Journal of Practical Medicine
2017;33(22):3694-3697
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function and molecular mechanism of tacrolimus in podocyte injury and restoration.Methods Cultured podocytes were stimulated by Angiotensin Ⅱ (Ang Ⅱ) or Ang Ⅱ plus tacrolimus.Cells were collected at different time points (0 h,12 h and 24 h).The distribution of F-actin was observed after immunofluorescence staining,and the protein expression of nephrin and podocin were detected by Western Blot (WB).Results In normal control podocytes,F-actin was arranged in cytoplasm powerfully.Ang Ⅱ induced the disruption and discontinuity of F-actin.Tacrolimus inhibited the effect of Ang Ⅱ,stabilized the regular arrangement the F-actin.Compared to normal cells,the protein expression of nephrin in Ang Ⅱ group significantly decreased at 24 h after stimulation (0.76 ± 0.32 in AngⅡ group vs.1.18 ± 0.40 in normal group,P ＜ 0.05).And tacrolimus stabilized the expression of nephrin protein (1.00 ± 0.19 in treatment group vs.0.76 ± 0.32 in Ang Ⅱ group,P ＜ 0.05).Ang Ⅱ and tacrolimus did not affect the expression of podocin protein.Conclusion Tacrolimus inhibits podocyte injury induced by Ang Ⅱ,stabilizes the regular arrangement of cytoskeleton and protein expression of nephrin.
