Effects of SNCG gene silencing on the cell cycle of endometrial carcinoma HEC-1A cells
10.3969/j.issn.1006-5725.2017.22.002
- VernacularTitle:γ-神经突触核蛋白基因沉默对子宫内膜癌HEC-1A细胞周期的影响
- Author:
Shaohua CHEN
1
;
Yujuan FAN
;
Wenyi LI
;
Hong XU
;
Hong CHEN
;
Jiangtao FAN
Author Information
1. 广西医科大学第一附属医院妇科
- Keywords:
endometrial carcinoma;
SNCG;
laser scanning confocal microscopy;
cell cycle
- From:
The Journal of Practical Medicine
2017;33(22):3686-3689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe changes of cell cycle after stable silencing of SNCG gene in human endometrial carcinoma HEC-1A cells.Methods Flow cytometry was used to observe the changes of SNCG stable silencing HEC-1A cell growth cycle.The percentage of G2/M phase of HCE-1A cells after acridine orange staining was observed by laser scanning confocal microscopy.Results Flow cytometry results showed that the group of SNCG stable silencing in G1 and G2/M phase of the cells increased,but the percentage of cells in the S phase were decreased,the difference were both statistically significant (P ＜ 0.05).The results of cells after confocal microscopy observation of acridine orange staining,the group of SNCG stable silencing were observed in G2/M phase high percentage were statistically significant (P ＜ 0.05).Conclusion Two experimental methods commonly validated that the cell cycle of SNCG stable silencing on HEC-1A cells was arrested in G2/M phase,suggesting that SNCG gene is closely related to the cell growth cycle of endometrial carcinoma.
