The expression and methylation status of Dickkopf-3 gene in oral squamous cell carcinoma
10.3969/j.issn.1001-3733.2018.02.014
- VernacularTitle:口腔鳞状细胞癌中Dickkopf-3基因的表达及甲基化状态
- Author:
Suxin ZHANG
1
;
Xin ZHANG
;
Zhong CHEN
;
Tianke LI
;
Yang BAO
;
Jing ZHENG
;
Yuwen ZHANG
Author Information
1. 河北医科大学第四医院口腔科
- Keywords:
Oral squamous cell carcinoma(OSCC);
Dickkopf-3;
Wnt signaling pathway;
Methylation
- From:
Journal of Practical Stomatology
2018;34(2):211-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the expression of Dickkopf-3(DKK-3) mRNA in oral squamous cell carcinoma(OSCC) and the relationship between the promoter methylation status and the carcinogenesis of OSCC. Methods: The expression of DKK-3 gene in 51 cases of OSCC and corresponding normal mucosa tissue was detected by PT-PCR and methylation-specific PCR, respectively. The relationship between DKK-3 and the clinical pathological features of the patients was analyzed with SPSS 13. 0. Results: The expression level of DKK-3 in OSCC group was lower than that in the control(t =-12. 580, P＜ 0. 05). The methylation rate of DKK-3 gene promoter region in OSCC group was significantly higher than that in the control(χ2 = 19. 273, P＜ 0. 05). The mRNA expression level of DKK-3 gene in OSCC with methylation group was lower than that in the control(t =-2. 817, P＜ 0. 05). Conclusion: Down-regulation of DKK-3 gene expression and hypermethylation of promoter region are important mechanisms in the pathogenesis of OSCC. The hypermethylation of DKK-3 promoter may be the main cause of transcriptional silencing.
