Determination of residual chloroform in medical polylactic acid membrane by gas chromatography

VernacularTitle:气相色谱法测定医用聚乳酸薄膜中三氯甲烷残留量
Author: Lihua LV ¹ ; Hua HUANG ; Shiyin LU ; Zhiheng SU
Author Information

1. 广西医科大学附属口腔医院
Keywords: Gas chromatography; Chloroform; Electron capture detector(ECD); Polylactic acid membrane
From: Journal of Practical Stomatology 2018;34(1):30-32
CountryChina
Language:Chinese
Abstract: Objective: To establish a method using gas chromatography for the determination of the residual chloroform in the medical polylactic acid membrane. Methods: The direct aqueous injection gas chromatographic method was established for the determination using RESTEK RTX capillary-column chromatography with the electron capture detector(ECD) at 270 ℃ and with the column at 85 ℃,maintained for 3 min. The split ratio of sampling was 10∶1. The injector temperature was 200 ℃. The high purity nitrogen was used as the carrier gas with the flow of 1. 0 ml /min. The injection volume was 1 μl. Results: The calibration curve showed a good linearity within the range of 25-2 000 ng /ml (r = 0. 999 9). The limit of detection was 1. 50 ng /ml,and the limit of quantitation was 4. 62 ng /ml. The average recovery rate was 98. 35％,RSD = 1. 98％. Conclusion: Gas chromatography is sensitive and accurate for the determination of the residual chloroform in the medical polylactic acid membrane.