The influence of ultraviolet treated zirconia with different roughness on the function of osteoblasts
10.3969/j.issn.1001-3733.2018.01.005
- VernacularTitle:氧化锆表面紫外线处理对成骨细胞生物学行为的影响
- Author:
Xiaoqing HOU
1
;
Yudong HOU
;
Xin SUN
;
Long SUN
Author Information
1. 滨州医学院口腔学院
- Keywords:
Zirconium oxide;
Ultraviolet;
Osteoblast
- From:
Journal of Practical Stomatology
2018;34(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of UV treatment of different zirconia surfaces on the function of MC3T3-E1 osteoblasts. Methods: Disks of zirconia-based materials with smooth(S) and rough(R) surfaces were treated and with and without UV light(15 W) for 48 h,respectively. MC3T3-E1 cells were cultured on zirconia disks and divided into 4 groups: smooth group(S),UV treated smooth group(S-UV),rough group(R) and UV treated rough group(R-UV). Cell proliferation was assessed by MTT 3 and 5 days after culture. Alkaline phosphatase(ALP) activity was determined 3 and 7 days after culture. Cell mineralization was observed by Alizarin red dye staining 21 days after culture. The mRNA expression of OCN and OPG were examined by RT-PCR 7 and 14 days after culture. Results: Proliferation and mineralization of MC3T3-E1 cells in R-UV group were more than in other groups(P ＜ 0. 05). R groups showed higher ALP activity than S groups at day 7. R-UV treatment increased the mRNA expressions of OPG and OCN in a time-dependent manner(P ＜ 0. 05). Conclusion: Ultraviolet light-treated zirconia surface may promote the proliferation,mineralization and the expression of OCN and OPG of MC3T3-E1 cells.
