The effects of He-Ne laser on the expression of extracellular matrix and its regulatory factors in degenerated temporomandibular joint of rabbits
10.3969/j.issn.1001-3733.2018.01.004
- VernacularTitle:氦氖激光对退变颞下颌关节髁突软骨基质与基质调节因子表达的影响
- Author:
Kaiping MAO
1
;
Jing ZOU
;
Jie LI
;
Mengya JIANG
;
Taozhen GAO
;
Guofu HUANG
Author Information
1. 武汉市第一医院口腔科
- Keywords:
He-Ne laser;
Temporomandibular joint;
Col-2;
PRG-4;
MMP-13;
TIMP-1;
BMP-2
- From:
Journal of Practical Stomatology
2018;34(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of He-Ne laser on the expression of extracellular matrix and its regulatory factors in degenerated temporomandibular joint(TMJ) of rabbits. Methods: 40 New Zealand adult white rabbits were randomly divided into normal group,sham model group,TMJOA model group and laser treatment group(n = 10),the rats in treatment group were treated by He-Ne laser irradination at acupoints. The rats in each group were divided into 1 d and 11 d groups(n = 5). The animals were respectively killed 1 d and 11 d after operation and HE staining was used to observe the histomorpholy. The protein expression of extracellular matrix and its regulatory factors were examined by Western blot. Results: After He-Ne laser treatment,the fiber layer of condylar cartilage was slightly loose,part of the fiber was newly produced. The level of Col-2,PRG-4,TIMP-1,BMP-2 was up-regulated and the of MMP-13 was down-regulated in the 11 d treatment group. Conclusion: He-Ne laser irradiation on acupoints may up-regulate the expression of extracellular matrix (Col-2 and PRG-4) and its regulatory factors (TIMP-1 and BMP-2),down-regulate the expression of MMP-13.
