The effects of radiation on miRNA-21 expression and cell biology of salivary gland adenoid cystic carcinoma cells
10.3969/j.issn.1001-3733.2017.06.017
- VernacularTitle:放疗对人涎腺腺样囊性癌细胞的miRNA-21与细胞生物学行为的影响
- Author:
Yumeng HUANG
1
,
2
;
Ying WANG
;
Tao WANG
;
Yong LI
;
Ping LIU
;
Hongwei ZHAO
Author Information
1. 401147重庆,口腔疾病与生物医学重庆市重点实验室
2. 江苏大学附属医院
- Keywords:
Salivary gland;
Adenoid cystic carcinoma;
Radiation sensitivity;
miRNA-21
- From:
Journal of Practical Stomatology
2017;33(6):792-797
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of radiation on miRNA-21 expression and the biology of human salivary gland adenoid cystic carcinoma cell lines(SACC-83,SACC-LM).Methods:In vitro cultured SACC-83 and SACC-LM cells were radiated by Varian 23 EX with the dose of 0(control),2,6 and 10 Gy respectively.Cell proliferation,apoptosis and miRNA-21 expression were observed by CCK-8 assay,flow cytometry and qRT-PCR methods respectively.Results:48 h after radiation the proliferation ability of SACC-LM cells decreased with the increasing dose of radiation(F =1 321.646,P =0.000),so did the miRNA-21 expression (F =177.964,P =0.011).However,the early apoptosis rate (F =354.484,P =0.039),the later apoptosis rate (F =254.278,P =0.042) and heteroploid ratio(F =1 562.991,P =0.001) increased with the increasing dose of radiation.In SACC-83 cell line 48 h after 6 Gy radiation the cell proliferation(F =1 537.214,P =0.013) and the miRNA-21 expression(F =134.868,P =0.017) were lower than that of other radiation doses.Moreover,the early apoptosis rate (F =88.579,P =0.006),the late apoptosis rate (F =1 391.345,P =0.033),heteroploid ratio(F =250.461,P =0.004) were higher than that of other radiation doses.Conclusion:The miRNA-21 expression in SACC-LM and SACC-83 cell lines is conversely associated with the radiation sensitivity.
