The attachment and collagen deposition of human gingival fibroblasts on titanium surface with different topography
10.3969/j.issn.1001-3733.2017.06.013
- VernacularTitle:不同钛表面形貌对人牙龈成纤维细胞附着及胶原沉积的影响
- Author:
Weiyan MENG
1
;
Shuang ZHAO
;
Heling WANG
;
Qing CAI
;
Baosheng LI
;
Yanmin ZHOU
Author Information
1. 130021长春,吉林大学口腔医学院,吉林省牙发育及颌骨重塑与再生重点实验室
- Keywords:
Surface treatment;
Fibroblast;
Soft tissue interface;
Nanometer topography;
Micrometer topography;
Electrochemical etching
- From:
Journal of Practical Stomatology
2017;33(6):772-777
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the attachment and collagen deposition of human gingival firbroblasts (HGFs) on titanium surface with different topography.Methods:Titanium surfaces created by machining(group M),electrolytic etching(group ECE) and electrolytic etching and acid etching(group ECA) were observed by SEM.HGFs cultured on the titanium surfaces were observed by laser scanning confocal microscope.Attachment of the cells was examined by comparing the numbers of attached to detached cells,respectively.Collagen production and deposition were examined via a Sirius red-based stain assay and confocal laser scaning microscopy.Results:The surface rouphness (μm) of group M,ECE and ECA was 0.867 5 ± 0.136 8,1.749 8 ± 0.355 1 and 1.671 4 ± 0.297 0 (P＜ 0.05) respectively,Cell attachment was significantly weaker on machined surface than on ECE and ECA surfaces,while which was weaker on ECE surface than on ECA surface.Collagen production was the highest on the machined surface,followed by that on ECE and ECA surface,Collagen deposition displayed a parallel pattern on the machined surface,while it was multidirectional on the ECE and ECA surfaces.Conclusion:The ECA surface of titanium may be beneficial to HGFs attachment,the machined surface may promote collagen deposition.
