The oxidative damage on the proliferation of oral cancer SCC-25 cells
10.3969/j.issn.1001-3733.2017.06.011
- VernacularTitle:氧化损伤对SCC-25细胞增殖抑制的研究
- Author:
Jingyu TAN
1
;
Junjian CHEN
Author Information
1. 锦州医科大学
- Keywords:
Hydrogen peroxide;
Oxidative stress;
Inflammation
- From:
Journal of Practical Stomatology
2017;33(6):763-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the exogenous oxidative stimuli on the mtDNA damage and proliferation of oral cancer SCC-25 cells.Methods:SCC-25 cells and fibroblast cell line BJ cells were treated by H2O2 at 0 (the control),120,240,480 and 960 μmol/L respectively for 15 m-24 h respectively,mtDNA damage was measured by a supercoiling-sensitive qPCR method(ss-qPCR),cell proliferation was tested by MTT assay.Results:Dose-dependent mtDNA damage was observed in SCC-25 cells treated by H2O2 at 120 μmol/L or above,and in BJ cells by H2O2 at 240 μmol/L or above,the mtDNA damage rate of both cells decreased 2-24 h after H2O2 treatment,in the 120 μmol/L group the damage rate reached to the control group.BJ cells showed less reaction to H2O2 stimulation than SCC-25 cells.Conclusion:Oxidative stress may be related to the development of oral cancer.
