The effects of BMP-7 on the biological properties of MC3T3-E1 osteoblasts under high glucose concentration in vitro
10.3969/j.issn.1001-3733.2017.06.005
- VernacularTitle:BMP-7对高糖环境下成骨细胞生物学性能的影响
- Author:
Long SUN
1
,
2
;
Pengfei XUE
;
Xiaoqing HOU
;
Yu SONG
;
Yudong HOU
Author Information
1. 264003,滨州医学院口腔医学院
2. 洛阳拜博口腔医院
- Keywords:
Bone morphogenetic protein-7 (BMP-7);
High glucose;
Osteoblast;
Proliferation
- From:
Journal of Practical Stomatology
2017;33(6):735-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of bone morphogenetic protein 7 (BMP-7) on the biological properties of osteoblasts exposed to high glucose.Methods:MC3T3 cells were cultured in the high glucose condition and were divided into 4 groups according to BMP-7 concentration (ng/ml):0 (control group),50,100 and 200 ng/mL BMP-7 (test groups).The cell proliferation and ALP activity were examind 1,3,5 and 7 d after exposure.The number of intracellular calcium nodules were observed with Alizarin red staining after osteogenesis induction for 21 days.The F-actin cytoskeleton of MC3T3 was stained with Rhodamine and examined 24 h after culture.The mRNA expression of OCN and Runx2 were quantified by RT-qPCR 48h after exposure to BMP-7.Results:BMP-7 significantly promoted proliferation of MC3T3 cells and enhanced ALP activity(P ＜ 0.05),increased the number and volume of intracellular calcium nodules.In the cells exposed to BMP-7,the osteoblast form was improved and F-actin cytoskeleton started to change with network structure.Compared with control group,the mRNA levels of OCN and Runx2 were up-regulated in BMP-7 groups (P ＜ 0.05).The mRNA levels of OCN and Runx2 were the highest in 200 ng/ml BMP-7 group(P ＜ 0.05).Conclusion:BMP 7 may inhibit the action of the high concentration of glucose on MC3T3 cells.BMP-7 may be benificial to osseointegration of dental implant in patietents with diabetics.
