Diabetic conditions modulate the adenosine monophosphate-activated protein kinase of podocytes.
- Author:
Tae Sun HA
1
;
Hye Young PARK
;
Ja Ae NAM
;
Gi Dong HAN
Author Information
1. Department of Pediatrics, College of Medicine, Chungbuk National University, Cheongju, Korea. tsha@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Adenosine monophosphate-activated protein kinase;
Advanced glycosylation end products;
Diabetic nephropathy;
Podocyte
- MeSH:
Adenosine*;
AMP-Activated Protein Kinases;
Animals;
Blotting, Western;
Cytoplasm;
Diabetic Nephropathies;
Glucose;
Glycosylation End Products, Advanced;
Metformin;
Mice;
Natural Resources;
Phosphorylation;
Podocytes*;
Protein Kinases*;
Proteinuria;
Rats
- From:Kidney Research and Clinical Practice
2014;33(1):26-32
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Adenosine monophosphate-activated protein kinases (AMPKs), as a sensor of cellular energy status, have been known to play an important role in the pathophysiology of diabetes and its complications. Because AMPKs are known to be expressed in podocytes, it is possible that podocyte AMPKs could be an important contributing factor in the development of diabetic proteinuria. We investigated the roles of AMPKs in the pathological changes in podocytes induced by high-glucose (HG) and advanced glycosylation end products (AGEs) in diabetic proteinuria. METHODS: We prepared streptozotocin-induced diabetic renal tissues and cultured rat and mouse podocytes under diabetic conditions with AMPK-modulating agents. The changes in AMPKalpha were analyzed with confocal imaging and Western blotting under the following conditions: (1) normal glucose (5mM, =control); (2) HG (30mM); (3) AGE-added; or (4) HG plus AGE-added. RESULTS: The density of glomerularphospho-AMPKalpha in experimental diabetic nephropathy decreased as a function of the diabetic duration. Diabetic conditions including HG and AGE changed the localization of phospho-AMPKalpha from peripheral cytoplasm to internal cytoplasm and peri- and intranuclear areas in podocytes. HG reduced the AMPKalpha (Thr172) phosphorylation of rat podocytes, and similarly, AGEs reduced the AMPKalpha (Thr172) phosphorylation of mouse podocytes. The distributional and quantitative changes in phospho-AMPKalpha caused by diabetic conditions were preventable using AMPK activators, metformin, and 5-aminoimidazole-4-carboxamide-1beta-riboside. CONCLUSION: We suggest that diabetic conditions induce the relocation and suppression of podocyte AMPKalpha, which would be a suggestive mechanism in diabetic podocyte injury.
