Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment.
- Author:
Dae Young YOO
1
;
Woosuk KIM
;
Dae Won KIM
;
Sung Min NAM
;
Hyo Young JUNG
;
Jong Whi KIM
;
Choong Hyun LEE
;
Jung Hoon CHOI
;
Moo Ho WON
;
Yeo Sung YOON
;
In Koo HWANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: brain-derived neurotrophic factor; dentate gyrus; high-fat diet; rosiglitazone
- MeSH: Animals; Blotting, Western; Brain-Derived Neurotrophic Factor/metabolism; Cell Differentiation/*drug effects; Cell Proliferation/drug effects; Dentate Gyrus/growth & development/physiology; Diet, Fat-Restricted; *Diet, High-Fat; Hippocampus/growth & development/physiology; Hypoglycemic Agents/*pharmacology; Immunohistochemistry; Ki-67 Antigen/metabolism; Male; Mice, Inbred C57BL; Microtubule-Associated Proteins/metabolism; Neurogenesis/*drug effects; Neuropeptides/metabolism; Thiazolidinediones/*pharmacology
- From:Journal of Veterinary Science 2014;15(1):27-33
- CountryRepublic of Korea
- Language:English
- Abstract: In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.
