Effect and mechanism of Solanine on proliferation and apoptosis of Jurkat cells
10.3760/cma.j.issn.2095-428X.2018.21.011
- VernacularTitle:龙葵碱对Jurkat细胞增殖、凋亡的作用及机制
- Author:
Yingxue LIU
1
;
Xiuhong JIA
;
Yingjie YI
;
Cong ZHU
Author Information
1. 256603,滨州医学院附属医院儿科
- Keywords:
Solanine;
Jurkat cell;
Proliferation;
Apoptosis
- From:
Chinese Journal of Applied Clinical Pediatrics
2018;33(21):1649-1651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of proliferation and apoptosis of Solanine on acute T lymphocyte leukemia (T-ALL) Jurkat cells and its mechanism.Methods After treated with different concentrations of Solanine,the proliferation of Jurkat cells was detected by CCK-8 assay,and the effect of Solanine on apoptosis of Jurkat cells were detected by flow cytometry.The expression of Bcl-2 and Bax in Jurkat cells were detected by Western blot,and the expression levels of Bcl-2 mRNA and Bax mRNA were detected by real-time fluorescence quantitative polymerase chain reaction.Results CCK-8 assay showed that Solanine significantly inhibited the proliferation of Jurkat cells in a dose-and time-dependent manner.The results of flow cytometry showed that the apoptosis rates of Jurkat cells treated with Solanine for 24 h were (2.40-± 0.98) ％,(28.43-± 4.86) ％,(41.56-± 1.87) ％,respectively,in a dose-dependent manner.Western blot showed that Solanine could increase the expression of Bax and decrease the expression of Bcl-2 in Jurkat cells,and they all were dose-dependent.Conclusion Solanine can significantly inhibit the proliferation and induce apoptosis of Jurkat cells.The mechanism is related to the up-regulation of Bax expression and down-regulation of Bcl-2 expression.
