Establishing A Method to Rapidly Remove Glycerol in Frozen Red Blood Cells
10.3969/j.issn.1671-7414.2018.02.029
- VernacularTitle:快速去除冰冻红细胞甘油的方法改进研究
- Author:
Na FENG
1
;
Jing LAN
;
Peng PENG
;
Jin WANG
;
Na LIU
;
Yuan ZHANG
;
Jing-Wen ZHAO
Author Information
1. 陕西省血液中心/西安市中心血站
- Keywords:
remove glycerol;
frozen red blood cells;
three-step method
- From:
Journal of Modern Laboratory Medicine
2018;33(2):105-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to rapidly remove glycerol in frozen red blood cells(RBC).Methods The glycerol in frozen RBC was removed by using three-step method as following:the frozen RBC were diluted by adding salt solution with high concentration.The salt solution was removed through centrifugation.The RBC were resuspended in normal saline of corresponding volume.Three-step method could acbieve the quick removal of glycerol by controlling tbe concentration salt water and action time.The indexes of hemoglobin,free hemoglobin,glycerol residue,residual white blood cell(WBC) and hematocrit of frozen-deglycerolized RBC were compared between conventional and improved methods.Results The traditional method to remove glycerol needed 1h approximately,while the three step method only needed 15 mins approximately,greatly shortening the time and improving the efficiency.The related tests for removing glycerol RBC,except hemoglobin content was slightly lower than the "The quality requirements for whole blood and blood components (2012 edition)",the rest of them all meeted the requirements.Conclusion The three-step method has the advantages of short time and high cfficiency.However,it still needs to be further improved and optimized to make the quality of product meet the national standard.
