Processing and Analysis of cTnⅠ Detected by Heterophilic Antibodies Interference with Chemiluminescence
10.3969/j.issn.1671-7414.2018.02.028
- VernacularTitle:异嗜性抗体干扰化学发光方法检测cTnⅠ的处理和分析
- Author:
Jun LU
1
;
Xin-Qi CHENG
;
Song-Lin YU
;
Li-An HOU
;
Cheng JIN
;
Ting-Ting YOU
Author Information
1. 中国医学科学院北京协和医学院北京协和医院检验科
- Keywords:
cardiac troponin Ⅰ;
interference;
heterophilic antibody
- From:
Journal of Modern Laboratory Medicine
2018;33(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and deal with false positive results caused by heterophile antibody interference immunoas say.Methods A 65 year old male patient's plasma samples were processed by dilution method,heterophile antibody blockers and tested using different detection systems (Beckman,Siemens and Abbott),respectively.The results obtained by multi plied dilution and different detection systems as well as pre and post-processed by heterophilic antibody blockers were compared and analyzed.Results The Beckman system was used to detect plasma cTnⅠ in patients before dilution and multiplied diluted at 2,4 and 8 times,with the results of 4.60,4.03,3.45 and 2.62 ng/ml,respectively.The cTnⅠ results of the patient's plasma determined by Beckman,Siemens and Abbott system were 4.60,0.023 and 0.022 ng/ml,respectively.The plasma cTnⅠ results of before and after heterophilic antibody blockers processing determined by Beckman system were 4.60 and 0.106 ng/ml respectively.Conclusion This cTnⅠ assay of the patient's plasma was interfered by heterophile antibodies,and altering the detection system or utilizing heterophile antibody blockers can solve such interference effectively.
