Evaluation of LD BC-Ⅰ Blood Cell Image Automatic Analyzer in Analysis of the Nucleated Cells in Peripheral Blood Smear
10.3969/j.issn.1671-7414.2018.02.024
- VernacularTitle:LD BC-Ⅰ全自动血细胞图像分析仪对外周血有核细胞分类能力的验证评价
- Author:
Fei GAO
1
;
Jing TANG
;
Jing-Gang LI
;
Feng ZHANG
;
Li LONG
;
De-Hua CAO
;
Jian WANG
Author Information
1. 福建医科大学附属协和医院血液病研究所
- Keywords:
artificial microscopy;
nucleated cells;
LD BC-Ⅰ automatic blood cell image analyzer
- From:
Journal of Modern Laboratory Medicine
2018;33(2):86-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify and evaluate the clinical performance of LD BC-Ⅰ blood cell image automatic analyzer.Methods A total of 202 EDTA-Na2 anticoagulant blood specimens were collected at the hospital clinic randomly between October and December 2016.After wright staining,each specimen was examined by microscopy and automatic analyzer respectively,the detection efficiency and the results of nuclear cells classification were compared between artificial microscopy and automatic analyzer,the correlation and consistency of two methods were further analyzed.Results The average time required for each specimen of the automatic analyzer was reduced by 3.81 minutes when compared with artificial microscopy,the P values was less than 0.01.LD BC-Ⅰ agreed 71.4％,64.8％,28.8％,21.1％ and 71.6％ respectively for pre-differentiation of the blast cells,promyelocytes,myelocyte,metamyelocytes and atypical lymphocytes.The diagnosis accordance rates of five nucleated cells above increased to 87.7％,81.5％,38.1％,26.3％ and 86.2％ after manual review.Passing Bablok regression analysis found that the correlation coefficient (r) of two methods of neutrophils was 0.981,lympho kjcytes (r =0.894),monocytes (r=0.725),eosinophils (r=0.772),and there were significant correlation between two methods (all P ＜0.01).Bland-Altman analysis found that the coincidence rate of the neutrophils and lymphocytes were 96％,the monocytes were 91％ and the eosinophils were 94％.Conclusion The LD BC-Ⅰ automatic blood cell image analyzer could significantly improve the analysis efficiency of the nucleated cells.There was good consistency in the classification results of mature granulocytes,monocytes and lymphocytes between instrumental detection and artificial microscopy.The method has certain clinical value to be applied widely.
