Serum Caspase-3 and Caspase-9 Activity in Patients with Lubar Intervertebral Disc Protrusion
10.3969/j.issn.1671-7414.2018.02.005
- VernacularTitle:腰椎间盘突出症患者血清中Caspase-3和Caspase-9活性研究
- Author:
Ying-Lun ZHAO
1
;
Yuan MA
;
Sen MO
;
Dong-Ran LI
;
Jun WANG
;
Chong LIU
;
Xin-Li ZHAN
Author Information
1. 广西医科大学第一附属医院脊柱骨病外科
- Keywords:
lumbar intervertebral disc degeneration;
lubar intervertebral disc protrusion;
caspase-3;
caspase-9;
apoptosis
- From:
Journal of Modern Laboratory Medicine
2018;33(2):13-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between apoptosis promoting effector molecules caspase-3,caspase-9 and lubar intcrvertebral disc protrusion.Methods 99 of operation patients with lubar intervertebral disc protrusion in their mater nity ward were recruited.Among them,single segment of lubar intervertehral disc protrusion were 70 (Group A),more than one segments of lubar intervertebral disc protrusion were 29(Group B).In addition,40 unrelated healthy people from physi cal examination center were enrolled as controls.Enzyme linked immunosorbent assay (ELISA) was used to examine serum caspase-3 and caspase-9 levels in lubar intervertebral disc protrusion patients.Results The level of caspase-3 in control group,group A and group B,respectively were 11.24±0.41,14.31±0.67 and 17.43±1.86 pmol/L.The caspase-3 activity in each group was statistically significant difference (F=8.47,P＜0.01).The level of caspase-9 in control group,group A and group B respectively were 18.54±2.19,30.57±3.63 and 43.68±5.15 pmol/L.The caspase-9 activity in each group was statistically significant difference (F=7.85,P=0.001).Compared with control group,the caspasc 3 and caspase-9 ac tivity in group A (q=3.08.3.29,all P＜0.05),group B (q=5.78,4.50,all P=0.014) was statistically significant differ ence.The caspase-3 and caspase-9 activity in group A,group B was statistically significant difference (q=3.21,3.22,all P＜0.05).Conclusion The augment of caspase-3 and caspase-9 promoted apoptosis of lubar intervertebral disc protrusion.It was connected with quantity of protrusive segments.The more segments of protrusion,the higher caspase-3 and caspase-9 levels of examination would be.
