Experimental Study on the Up-Regulation of NF-kappa B and MMP-9 mRNA Level in HUVECs Stimulated by Serum from Children with Coronary Artery Lesions of Kawasaki Disease

VernacularTitle:川崎病并发冠脉损伤患儿血清活化HUVECs NF-κB促使MMP-9 mRNA水平上调的实验研究
Author: Li-Sha LIU ¹ ; Zhe-Qian LI ; Cheng TAN ; Han-Qing YU
Author Information

1. 南京医科大学附属儿童医院检验科
Keywords: kawasaki disease; coronary artery lesions; nuclear factor-kappa B; matrix metalloproteases 9
From: Journal of Modern Laboratory Medicine 2018;33(1):131-132,136
CountryChina
Language:Chinese
Abstract: Objective To investigate the relationship between nuclear factor kappa B(NF-κB) and matrix metalloproteinase-9 (MMP-9) mRNA in human umbilical vein endothelial cells(HUVECs) stimulated by the serum from children with coronary artery lesions of Kawasaki disease (KD).Methods HUVECs were cultured and were divided into 4 groups:normal serum group,general fever group,Non-CALs group and CALs group.Co-Immunoprecipitation (ChIP) was used to detect the relationship between NF-κB and MMP-9,and RT-PCR was used to detect the mRNA level of MMP-9.Results Compared with control groups,NF-κB p65 could bind the promoter of MMP 9 in HUVECs cultured with 10 ％ serum from KD patients with coronary artery lesions.The mRNA level of MMP 9 was also up-regulated.Conclusion NF-κB p65 can promote the transcription of MMP-9 in HUVECs induced by the serum from KD patients with coronary artery lesions.