Comparative Study on Different Sample Pre-enrichment Methods for Flow Cytometry Cell Sorting
10.3969/j.issn.1671-7414.2018.01.033
- VernacularTitle:应用流式细胞技术分选单核细胞亚群不同预富集方法的探讨
- Author:
Hong-Yan YANG
1
;
Li-Fang YAN
;
Ru-Gang WANG
;
Xin ZHOU
Author Information
1. 北京市预防医学研究中心
- Keywords:
flow cytometry;
monocytes;
pre-enrichemnt
- From:
Journal of Modern Laboratory Medicine
2018;33(1):128-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different pre-enrichment methods on flow cytometry cell sorting of monocyte.Methods Peripheral blood of 15 volunteers were pre-enriched by three methods:erythrocyte lysis,hydroxyethyl starch sedimentation,density gradient centrifugation using Ficoll.Cell viability was detected by Trypan blue staining.The amount and the proportion of monocytes were analyzed by flow cytometry.Results The cell viability of the samples prepared by the 3 methods was above 95 ％.There was no significant difference between the absolute count and grouping of monocytes in erythrocytes lysis and without pre-enrichment samples.The cell subgroup was not obvious by hydroxyethyl starch sedimentation.The number of monocytes obtained by Ficoll density gradient centrifugation was significantly lower than that without pre-enrichment samples.Conclusion Erythrocyte lysis was a high efficient method for monocytes flow cytometry cell sor ting.
