High Expression of CD36 in Peripheral Blood Mononuclear Cells from Patients with Crytococcal Meningitis and Its Clinical Significance
10.3969/j.issn.1671-7414.2018.01.014
- VernacularTitle:CD36在隐球菌脑膜炎患者外周血单个核细胞中表达升高及其临床意义
- Author:
Teng-Da LI
1
;
Yuan-Lan HUANG
;
Shu-Ping LONG
;
Yun LIU
;
Peng LIU
;
Wei-Wei ZHANG
;
Jie GUO
;
Ming-Li GU
;
An-Mei DENG
Author Information
1. 第二军医大学长海医院,上海200433
- Keywords:
crytococcal meningitis;
CD36;
IFN-γ;
TGF-β
- From:
Journal of Modern Laboratory Medicine
2018;33(1):56-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression level of CD36 in peripheral blood mononuclear cells(PBMCs) from patients with crytococcal meningitis and its clinical significance.Methods The experimental group was the peripheral blood samples from 36 patients diagnosed as crytococcal meningitis in Changhai Hospital and Changzheng Hospital in Shanghai from September 2013 to February 2017,while the control group was the peripheral blood samples from 36 health individuals examined at the same time.PBMCs were separated by density gradient centrifugation method,the relative mRNA expression of CD36,TLR4 in PBMCs was tested by qRT-PCR,the expression level of cytokines such as TNF-α in plasma was tested by ELISA,the comparison of the examined values of experimental and control groups performed by two independent samples' t test,Pearson correlation analysis was performed on the relative mRNA expression of CD36 in PBMCs and cytokines such as TNF-α in experimental group.Results The relative mRNA expression of CD36 in PBMCs of experimental group was 2.01 ± 0.63,the control was 1.49 ± 0.47,and there was statistical difference (t =3.969,P =0.000 2).Pearson analysis results showed that in experimental group CD36 was positively related with the protein expression level of IFN-γ in plasma (r=0.384,P＜0.05) and negatively related with TGF-β(r=-0.487,P＜0.005).Conclusion CD36 might involve in the immunopathology process of crytococcal meningitic by influencing the expression of cytokines such as IFN-γ,TGF-β,and was the potential target for monitoring and treating this disease.
