Detection and Analysis of Candida Albicans Enolase Antigen-Specific T Cells in Heathy Individuals by ELISPOT Assay
10.3969/j.issn.1671-7414.2018.01.003
- VernacularTitle:健康人外周血白念珠菌烯醇化酶抗原特异性T细胞检测
- Author:
Xi-Min JIANG
1
;
Bin ZHANG
;
Li-Ning SHI
;
Xiu-Juan SHANG
;
Cai-Yun ZHANG
;
Jing-Yu WANG
;
Yu-An HU
Author Information
1. 中国人民解放军南京总医院医学信息科
- Keywords:
enolase;
ELISPOT;
interferon gamma;
interleukin-4;
interleukin-17A;
candida albicans
- From:
Journal of Modern Laboratory Medicine
2018;33(1):10-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the T cells immune response to Enolase (Eno),an immunodominant antigen of Candida albicans.Methods Determined the frequencies of positive spot-forming cells (SFCs) of Eno antigen-specific T cells secreting IFN-γ,IL-4 and IL-17A in the PBMCs of 25 healthy individuals by ELISPOT assay.Results After Eno stimulation,the SFCs of IFN-γ,IL-4 and IL 17A in 25 healthy persons were 14.00(8.50,39.00),0(0,0) and 2(1,4.50),respectively.Either the SFCs of IFN-γ or those of IL-17A were significantly higher than those of IL-4 (P＜0.05).The difference between SFCs of IFN-γ and those of IL-17A was also significant (P=0).The response rates of IFN-γ,IL-4 and IL-17A were 100％ (25/25),4.00％ (1/25) and 88.00％ (22/25),respectively.The difference between either IFN-γor IL 17A and IL-4 was significant (values all P＜0.05).Eno induced strong response (SCFs≥20) for IFN-γ in 10 healthy individuals (40.00％,10/25),but failed to induce strong response for IL-17A and IL-4 in all the volunteers.Major healthy individuals (84.00％,21/25)showed both Th1 and Th17 cells response against Eno,12.00％ (3/25) showing Th1 cells response in isolation,and none showed Th2 or Th17 cells response individually.Conclusion Eno of Candida albicans could induce immunodominant responses of Th1 and Th17 cells,which was considered to provide protection to IC.Eno might be a potential protective vaccine against IC.
