Study on Effect of Qing-Chang Wen-Zhong Decoction on Interferon Gamma Induced Protein 10 in Ulcerative Colitis Rats
- VernacularTitle:清肠温中方对溃疡性结肠炎大鼠干扰素γ诱导蛋白10的影响
- Author:
Tangyou MAO
1
;
Rui SHI
;
Tianhong XIE
;
Yi GUO
;
Chen CHEN
;
Lei SHI
;
Boyi JIA
;
Jiali LIU
;
Xiang TAN
;
Yafei HAN
;
Panghua DING
;
Junxiang LI
Author Information
- Keywords: Qing-Chang Wen-Zhong decoction; ulcerative colitis; interferon gamma inducible protein 10; regulation effect
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(11):1836-1840
- CountryChina
- Language:Chinese
- Abstract: This paper was aimed to study the effect of Qing-Chang Wen-Zhong (QCWZ) decoction on interferon gamma induced protein 10 (IP10) in colon tissues of rats with ulcerative colitis (UC).The UC model was induced using 4.5％ DSS added to distilled water for 7 days.At the same time,low-,medium-and high-dose of QCWZ decoction and mesalazine was given by gavage route daily.Then,the rats were killed and the colon tissues were taken.Expression level of interleukin-1 alpha (IL-1α),IL-1β,IL-6,tumor necrosis factor alpha (TNF-α) and interferon gamma (INF-γ) in colon were detected by Elisa assay.The expression and distribution of IP10 protein were detected by immunohistochemistry (IHC).The results showed that compared with the normal group,inflammatory factors (IL-1α,IL-1β,IL-6,TNF-α,INF-γ) and IP10 expression level in DSS-induced UC rats were significantly increased.After 7 days of intervention,inflammatory factors (IL-1α,IL-1β,IL-6,TNF-α,INF-γ) and IP10 decreased significantly (p＜0.01,p＜0.05).It was concluded that QCWZ decoction may down-regulate the expression of IP 10 and inflammatory factors (IL-1α,IL-1β,IL-6,TNF-α,INF-γ),and then inhibit intestinal inflammation and repair intestinal mucosal damage,so as to achieve the purpose of UC treatment.
