Correlation Analysis on Cross Section Color of Licorice and Its Active Ingredients
10.11842/wst.2017.11.011
- VernacularTitle:甘草断面颜色与其有效成分的相关性分析
- Author:
Wenjuan MI
1
;
Xianglong LIN
;
Yang LI
;
Huirong CHEN
;
Yonghong YAN
;
Huiqin ZOU
Author Information
1. 北京中医药大学中药学院 北京 102488
- Keywords:
Colorimeter;
licorice;
cross section color;
active ingredient;
correlation
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(11):1829-1835
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish an objective and convenient method to evaluate the quality of licorice through the study on correlation between the cross section color and contents of active ingredients of licorice.Therefore,colorimeter was introduced and applied to determinate cross section color of licorice.Meanwhile,contents of five active ingredients of licorice were also determined.HPLC was used to determine liquiritin and glycyrrhizic acid.Colorimetric method was used to determine total saponins.Ultraviolet spectrophotometry was used to determine total flavonoids.Sulphuric acid-phenol colorimetry was used to determine polysaccharides.Correlation between the cross section color and content determination result was analyzed.The results showed that the correlation coefficient of glycyrrhizic acid content and L* was-0.578,P ＜ 0.001,the correlation coefficient with b* was 0.596,P ＜ 0.001;the correlation coefficient of liquiritin content and L* was-0.503,P =0.002,the correlation coefficient with b* was 0.890,P ＜ 0.001;the correlation coefficient of total flavonoids content and L* was-0.729,P ＜ 0.001,the correlation coefficient with b* was 0.724,P ＜ 0.001;the correlation coefficient of polysaccharides content and L* was 0.230,P =0.190,the correlation coefficient with b* was-0.390,P =0.023;the correlation coefficient of total saponins content and L* was-0.411,P =0.016,the correlation coefficient with b* was 0.738,P ＜ 0.001.It was concluded that the cross section color index of licorice has significant correlation with contents of glycyrrhizic acid,liquiritin,total flavonoids and total saponins.There was no significant correlation with content of polysaccharides.It illustrated the close correlation between cross section color of licorice and its active ingredients.Through the digitalized determination on color,contents of chemical composition in licorice can be initially determined or predicted objectively.It provided a new idea and method for the quality evaluation of Chinese herbal medicine.
