Effects of Sinomenine on Tumor Suppressor Gene P16 And P53 in Rats with Lung Cancer
10.11842/wst.2017.10.019
- VernacularTitle:青藤碱对肺癌模型大鼠抑癌基因P16和P53的影响
- Author:
Huajun ZHENG
1
;
Xianhe WANG
;
Changbin KE
;
Li LI
Author Information
1. 湖北医药学院附属人民医院 十堰442000
- Keywords:
Sinomenine;
P16;
P53;
transplanted lung cancer;
cell cycle;
tumor inhibition rate
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(10):1702-1706
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of sinomenine on the expression of tumor suppressor gene P 16 and P53 in rats with lung cancer.A total of 40 male SD rats were treated by left-lung vein injection of WALKER-256 cell suspension to establish transplanted lung cancer model.After 3 weeks,30 rats screened of tumor were randomly divided into the model group,cyclophosphamide (CP) group and the sinomenine treatment group.Another 10 healthy SD rats were set as the normal control group.Sinomenine treatment group was treated with the subcutaneous injection of 10％ sinomenine hydrochloride for 10 weeks.CP was injected in the CP group as positive control.The same amount of normal saline was injected in the normal control group and the model control group.After 10 weeks of treatments,lung tumors of each group were removed to measure the tumor volume and weight.And the tumor inhibition rate was calculated.Then,flow cytometry was used to detect the proportion of WALKER-256 cells in tumor tissues in G1,G2,M and S around four cycles.Immunohistochemistry was adopted to detect positive expression rates of P16 and P53 protein.Reverse transcription polymerase chain reaction (RT-PCR) were used to detect expression of P16mRNA and P53mRNA.The results showed that compared with the model control group,the inhibition rate of sinomenine group was 30.15％;the positive expression rate of P16mRNA and P53mRNA protein were significantly decreased;expressions of P 16mRNA and P53mRNA were lower;tumor volume and tumor weight in S period got down significantly.The rates of cells in G1 and G2 periods got higher (P＜0.05).It was concluded that sinomenine may inhibit the differentiation and proliferation of WALKER-256 transplanted lung cancer cells in rats by regulating the expression of tumor suppressor gene P 16 and P53,regulating the ratio of cells in G1,G2 and S periods.
