Simultaneous Determination of Five Components in Baihu and Guizhi Decoction by HPLC
- VernacularTitle:高效液相色谱法同时测定白虎加桂枝汤中5个有效指标性成分的含量
- Author:
Ping PENG
1
;
Mengjie WANG
;
Xiaoying LI
;
Xiangning DUAN
;
Yiteng LI
;
Jing DU
Author Information
- Keywords: Baihu decoction; mangiferin; liquiritin; cinnamic acid
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(8):1391-1395
- CountryChina
- Language:Chinese
- Abstract: This study was aimed to develop an HPLC method for simultaneous determination of five active components in Baihu and Guizhi decoction.Simultaneous determination of mangiferin,neomangiferin,glycyrrhizic acid,liquiritin,and cinnamic acid in Baihu and Guizhi decoction were conducted by HPLC-PDA under multiple UV wavelengths.An Waters xBridge BEH C18 Column (4.6 mm × 250 μm,5 μm) was used.The mobile phase was acetonitrile-0.01％ formic acid solution.The flow rate was 1 mL·min-1.The column temperature was kept at 24℃.The detection wavelength was set at 255 nm and 280 nm.The results showed that the linear range of mangiferin,neomangiferin,glycyrrhizic acid,liquiritin,and cinnamic acid was 66-1 976 μg (r =0.999 3),70-3487 μg (r =0.999 7),30-913 μg (r =0.999 5),35-1 734 μg (r =0.999 5),0.6-187 μg (r =0.999 8) in 70 min,respectively.The average recovery was between 95.29％ and 100.17％.It was concluded that the method was convenient,stable,reliable and accurate for simultaneous determination of the five components in Baihu and Guizhi decoction.It provided an evaluation method for the quality control of Baihu and Guizhi decoction and the research o its granules.
