Quality Evaluation of Terminalia billerica (Gaertn.) Roxb.Tannins Fraction from Different Habitats by HPLC Fingerprint Based on HPLC-MSn and Chemometrics
10.11842/wst.2017.07.024
- VernacularTitle:基于HPLC-MSn和化学计量学的指纹图谱在毛诃子鞣质部位质量评价中的应用
- Author:
Wenjing CHEN
1
;
Wenyi LIANG
;
Shi LI
;
Lingfang WU
;
Yaping CUI
;
Qi QI
;
Ting YE
;
Linjin LIANG
;
Lanzhen ZHANG
Author Information
1. 北京中医药大学中药学院 北京 100102
- Keywords:
Terminalia billerica (Gaertn) Roxb.;
tannin parts;
chemometrics;
HPLC;
fingerprints
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(7):1228-1233
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to establish the HPLC fingerprint of T.Billerica (Gaertn.) Roxb.tannin fraction.The analysis was performed on Atlantic T3 (250 mm × 4.6 mm,5 μm) C18 column.The mobile phase was acetonitrile-0.2％ glacial acetic acid aqueous solution with gradient elution.The flow rate was 1.0 mL·min-1.The injection size was 20 μL.The temperature was maintained at 30℃.Eleven batches of T.Billerica (Gaertn.) Roxb.tannin fraction of chromatographic data was analyzed by similarity of chromatographic data,SPSS software and SIMCA software.There were 20 common peaks in the diagram.The similarity analysis of 11 samples revealed that the similarities were between 0.832 and 0.973.Only the similarity of tannin parts from Xinjiang was below 0.9.The cluster analysis classified the tannin parts into 3 types,which shared the similar results as the principal components analysis (PCA).PLS-DA found that peak 1,13 and 14 may be the main chromatographic peaks to identify tannins fraction.The HPLC-MSn information of 14 compounds in T.billerica (Gaertn.) Roxb.was summarized.It was concluded that chemometrics analysis method can be used to analyze the HPLC fingerprint of T.billerica (Gaertn.) Roxb.tannin fraction.This method was rapid,simple,and reproducible.It can be used as one of the effective methods for the quality control of T.billerica (Gaertn.) Roxb.tannin fraction.
