Effects of Si-Wu Mixture on Ovarian Granulose Cell Treated with Cisplatin through TGF-β3 Protein Pathway
- VernacularTitle:四物合剂通过TGF-β3蛋白通路干预顺铂作用颗粒细胞E2分泌机制的研究
- Author:
Xinyue CAI
1
;
Piwen ZHAO
;
Binghua TANG
;
Xiaomin YANG
;
Hongbo WU
;
Lixia CUI
;
Liping SUN
Author Information
- Keywords: Si-Wu mixture; CYP19a1; TGF-β3; protein pathway; granulosa cells; estrogen
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(4):653-657
- CountryChina
- Language:Chinese
- Abstract: This study was aimed to investigate the effects of Si-Wu mixture on the secretion of E2 and the expression of CYP19al gene in granulosa cells after cell injury.Ovarian granulosa cells of SD rats were treated with cisplatin (CDDP).And the expression levels of E2 and CYP19a1 were determined by different testing methods.The results showed that the level of E2 induced by radioimmunoassay of the Si-Wu group was significantly higher than that of the CDDP group.Meanwhile,the group which added TGF-β3 protein pathway blocker was lower than others.The results of immunohistochemistry and western blotting showed that the expression level of CYP 19a1 of the Si-Wu group was higher than that of the CDDP group.In the western blotting,the group which added blocker was significantly lower than the non-blocking group.It was concluded that the pharmacological serum of Si-Wu mixture can enhance the level of E2 in CDDP cells through TGF-β3 protein pathway.And the effect is accomplished by the intervention of CYP 19a1.
