Separation and Identification of Surface Preponderant Fungi on Citri Reticulatae Pericarpium and Its Influence on Effective Substance
- VernacularTitle:陈皮表面优势真菌的分离鉴定及其对药效物质的影响
- Author:
Sujuan LIU
1
;
Xin ZHANG
;
Zhilei WANG
;
Yi HE
;
Qiuhua PU
;
Lin CHEN
;
Hongping CHEN
;
Youping LIU
Author Information
- Keywords: Citri reticulatae pericarpium; preponderant fungi; separation and identification; inoculation; effective substance
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(4):618-622
- CountryChina
- Language:Chinese
- Abstract: This paper studied the influence ofsurface preponderantfungi on main effective substances of CitriReticulatae Pericarpium (CRP).Spreading plate method was used to isolatepredominant strains from the surface of CRP.Besides,the method combining microscopic and molecular identification was also adopted.HPLC and UVspectrophotometric methods were used to determine the main effective substances in CRP.From thesurface of CRP,the advantage strain fungi were Aspergillusniger and A.Flavus.After the inoculation of A.Niger and A.flavus against CRP,the effective compositionwas changed.And different A.niger strains had differenceeffectiveness oneffective chemical components,especially one strain of A.niger.Compared withthe control group,contents of total flavonoids and hesperidin were significantly increased (P＜0.01);and five types of obvious new chemical compositions were produced.It was concluded that the metabolic transformation of fungi was related tochanges ofeffective substances of CRP,which played a significant role in the aging process of CRP.The growth and metabolism of fungi consumeeffeetive substances and producechanges of composition.From the perspective of microorganisms,"the older,the better" of CRP hasa better explanation.
