The relationship between interleukin-32 and gestational diabetes mellitus
10.3969/j.issn.1672-8467.2018.03.011
- VernacularTitle:白细胞介素-32与妊娠期糖尿病的相关性
- Author:
Zhi-Yan YU
1
;
Xin-Mei HUANG
;
Jun-Hua GUAN
;
Rui ZHANG
;
Min YANG
;
Yue-Yue WU
;
Bing-Bing ZHA
;
Jun LIU
Author Information
1. 复旦大学附属上海市第五人民医院内分泌科 上海200240
- Keywords:
interleukin-32;
diabetes mellitus;
gestation;
placenta;
umbilical cord;
adipose tissue
- From:
Fudan University Journal of Medical Sciences
2018;45(3):354-359,368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the concentration of interleukin-32 (IL-32) in serum,and the expression of IL-32 mRNA in placenta,subcutaneous fat and great epiploon adipose tissue,and explore the relationship between IL-32 and gestational diabetes mellitus (GDM).Methods The concentrations of serum IL-32 in 42 GDM (GDM group) and 38 non-GDM (control group) pregnant women were examined by enzyme linked immunosorbent assay (ELISA).The level of the IL-32mRNA in the placenta,subcutaneous fat and great epiploon adipose tissue was examined by quantitative realtime reverse-transcription polymerase chain reaction (RT-qPCR).The expression levels of IL-32 protein in the placenta and umbilical cord were measured by immunohistochemical staining (IHC) and analyzed by ImagePro Plus 9.0.Results The serum level of IL-32 in the GDM group was significantly higher than that in the control group [(129.3 ± 5.78) pg/mL vs.(105.6 ± 8.61) pg/mL,P＜0.05].The level of IL-32 mRNA was increased 1.32 and 1.66 fold in the placenta (P＜0.05) and the great epiploon adipose tissue (P＜0.05) from GDM women,compared with that from control group.No significant difference was found in the levels of IL-32mRNA in the subcutaneous adipose tissue between the two groups [(3.78 ± 0.53) vs.(3.61 ± 0.35),P＞0.05].The expression of IL-32 in the placenta from GDM group was 1.27 times higher than that from control group (P ＜0.05).The IL-32 in the umbilical artery and umbilical vein of GDM group increased by 1.30 and 1.32 (P＜0.05).But there was no significant difference of IL-32 expression in the umbilical interstitial between the two groups.Conclusions Overexpression of IL-32 in the serum and tissue may be involved in the pathogenesis of GDM.
