The role and clinical significance of αB-Crystallin in the proliferation and migration of non-small cell lung cancer
10.3969/j.issn.1672-8467.2018.03.006
- VernacularTitle:αB-Crystallin在非小细胞肺癌增殖迁移中的作用及其临床意义
- Author:
Jie GU
1
;
Feng-Kai XU
;
Qiao-Liang ZHU
;
Lin WANG
;
Di GE
;
Chun-Lai LU
Author Information
1. 复旦大学附属中山医院胸外科 上海200032
- Keywords:
non-small cell lung cancer;
αB-Crystallin;
proliferation;
migration
- From:
Fudan University Journal of Medical Sciences
2018;45(3):323-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of αB-Crystallin in non-small cell lung cancer and adjacent tissues (NSCLC) and analyze the associations between its expression and clinicopathological features as well as proliferation and migration of NSCLC cells.Methods We down-regulated the expression of αB-Crystallin in NSCLC cell line A549 with specific siRNA,and assessed the role of αB-Crystallin in the proliferation and migration of NSCLC cell line with CCK-8 and Transwell.Then,we examined αB-Crystallin expression by immunohistochemistry (IHC) in tissue microarray (TMA) consisting of 208 cases of NSCLC who were treated in the Department of Thoracic Surgery,Zhongshan Hospital in 2005.Associations between αB-Crystallin expression and clinical characteristics were assessed and the prognostic role of αB-Crystallin in NSCLC was evaluated by Kaplan-Meier and Cox regression analysis.Results Loss of αB-Crystallin expression suppressed the proliferation and migration potential of A549 cells (P＜0.05).Furthermore,immunohistochemistry in tissue microarray revealed that αB-Crystallin was overexpressed in NSCLC compared with adjacent normal tissues,αB-Crystallin expression was associated with lymph node metastasis (P ＜ 0.05).Multivariate analysis indicated that αB-Crystallin expression was an independent prognostic factor in patients' overall survival.Conclusions Our finding indicated that αB-Crystallin was overexpressed in NSCLC and promotes proliferation and migration of NSCLC cells.Its expression was associated with poor prognosis of NSCLC patients.αB-Crystallin may represent a potential therapeutic target and a novel prognostic marker of NSCLC.
