The role of Tif1γ on apoptosis of pancreatic cancer cells
10.3969/j.issn.1672-8467.2018.01.010
- VernacularTitle:Tif1γ在胰腺癌细胞凋亡中的作用
- Author:
Yan-Yuan TU
1
;
Wen-Hao TANG
;
Wei-Yi WANG
Author Information
1. 复旦大学附属华东医院普外科 上海200040
- Keywords:
Tif1γ;
pancreatic cancer cell;
apoptosis;
raltitrexed;
gemcitabine
- From:
Fudan University Journal of Medical Sciences
2018;45(1):62-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transcriptional internediary factor 1 gamma (Tif1γ)and its signal pathway related proteinson apoptosis of human pancreatic cancer cell line Capan-1 treated with either gemcitabine (GEM) or raltitrexed (RTX).Methods Capan-1 cells were treated with GEM of 559 μmol/L or RTX of 0.86 μmol/L for 36 h.The cell apoptosis of Capan-1 was determined using flow cytometry.Protein levels of Tif1γ,TGF-β1,Smad3,p-Smad3,Smad4,Bcl2,BAX and Caspase3 in Capan-1 cells were determined by Western blot.Results The late apoptosis and death ratio after RTX treatment were 28.7％ ± 5.1％ and 3.7％ ± 0.5％,respectively,showing significant difference from that after GEM treatment or untreated control group (P＜0.01).The results of Western blot showed that the relative protein levels of Tif1 γ,TGF-β1,p-Smad3,BAX and Caspase3 in Capan-1 cells were increased in RTX group compared with those in GEM group or control group (P＜0.05).The relative protein levels of Smad4 and the Bcl2/Bax ratio were decreased in RTX group compared with those in GEM group or control group (P＜0.05).Conclusions Increased level of Til1γ by RTX treatment resulted in decreased level of Smad4 to regulate the balance of Bcl2/Bax,increased Caspase3 and increased apoptosis in Capan-1 cells.
