Monitoring EGFR T790M mutations by Blocker PCR in plasma of advanced non-small-cell lung cancer patients with EGFR-TKI acquired resistance
10.3969/j.issn.1672-8467.2018.01.007
- VernacularTitle:改良Blocker PCR检测EGFR-TKI耐药后非小细胞肺癌血浆EGFR T790M突变的价值
- Author:
Mei-Ling ZHANG
1
;
Chun LI
;
Mao-Song YE
;
Zi-Ying GONG
;
Dao-Yun ZHANG
;
Xin ZHANG
Author Information
1. 复旦大学附属中山医院呼吸内科 上海200032
- Keywords:
cell free DNA;
EGFR T790M mutations;
Blocker PCR;
non-small-cell lung carcinoma
- From:
Fudan University Journal of Medical Sciences
2018;45(1):45-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of Blocker PCR assays in monitoring T790M mutations in plasma of non-small-cell lung cancer (NSCLC) patients with epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) acquired resistance.Methods Blocker PCR assays were employed to identify mutations in plasma for 127 advanced NSCLC with acquired EGFR-TKI resistance.In addition,the paired tumor re-biopsy or PE samples were obtained to analyze EGFR mutations.Meanwhile,we evaluated the detection accuracy of Blocker PCR assays in comparison with the next generation sequencing (NGS).Results Among the 127 patients,40.15% (51/127) EGFR T790M was detected in the plasma,78.44% (40/51) coexisted with an EGFR activating mutation.Additionally,54.54 % (6/11) EGFR T790M was identified in re-biopsy tissues,while 43.75 % (14/32) were detected in the plasma.Furthermore,the concordance rate of Blocker PCR and NGS in identifying EGFR sensitizing mutations and EGFR T790M mutations was 100%.Conclusions Blocker PCR is a highly sensitive and reliable method in monitoring EGFR T790M mutations in the plasma of NSCLC patients with EGFR-TKI acquired resistance.