Antibody Response of Mouse Splenocytes Using Mixture of Supernatants of Thymocytes, Adherent and Non-adherent Splenocytes: In-vitro Immunization-II.
- Author:
Dongsoo KIM
1
;
Geun Woong NOH
;
Duk Hi KIM
;
Oh Hun KWON
Author Information
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords: In-vitro immunization; ELISA; Growth hormone
- MeSH: Animals; *Antibody Formation; Antigens/immunology; Cell Adhesion; Female; Growth Substances/*immunology; Immunization; Lymphocyte Activation/immunology; Mice; Mice, Inbred BALB C; Recombinant Proteins/immunology; Spleen/cytology/*immunology; T-Lymphocytes/*immunology
- From:Journal of Korean Medical Science 1990;5(1):25-31
- CountryRepublic of Korea
- Language:English
- Abstract: We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported several methods of in-vitro immunization using different conditioned media. Here we introduce another method of in-vitro immunization using a mixture of three types of supernatant (thymocytes, and adherent and non-adherent splenocytes of mouse). Splenocytes were immunized in-vitro by a recombinant human growth hormone (rhGH) with the above conditioned media, and the results by ELISA showed a much higher optical density than the other in-vitro immunization methods that we had previously reported. Humoral responses as a result of in-vitro immunization to soluble antigens were easily confirmed by ELISA using the above-conditioned media. This finding indicates that any other conditions thought to be critical by other researches may not be essential for in-vitro immunization.
