Effect of miR-196 on endocrine therapy for breast cancer by targeting p27

VernacularTitle:miR-196调控p27表达对乳腺癌细胞内分泌治疗耐药的影响
Author: Ran MENG ¹ ; Yue YU ; Xuchen CAO
Author Information

1. 300060 天津医科大学肿瘤医院乳腺一科,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,教育部乳腺癌防治重点实验室
Keywords: Breast cancer; Endocrine therapy; miR-196; p27
From: Chinese Journal of Endocrine Surgery 2017;11(6):504-508
CountryChina
Language:Chinese
Abstract: Objective To determine the effect of miR-196 on sensitivity of endocrine therapy for breast cancer and to explore its possible molecular mechanism.Methods The expression of miR-196 was detected by real-time quantitative PCR (RT-qPCR) in MCF7 (ER+) and BT549 (ER-).The sensitivity of tamoxifen on MCF7 cells was evaluated by MTT and colony formation.Dual-luciferase,RT-qPCR and western blot assays were used to determine the regulation of miR-196 on p27.Results The expression of miR-196 was up-regulated in BT549 compared to that in MCF7 cells.The cell viability and colony formation were increased in miR-196-overexpressed MCF7 cells compared to those in the control cells after treatment with tamoxifen.The luciferase activity and expression of p27 were decreased in miR-196-overexpressed MCF7 cells compared to those in the control cells.Overexpression of p27 eliminated the effect of miR-196-induced endocrine therapy resistance in MCF7 cell.Conclusion miR-196 promotes breast cancer endocrine resistance by targeting p27.