Expression of hormone sensitive lipase and carnitine acyl transferase-1 in obese diabetic rats after sleeve gastrectomy
10.3760/cma.j.issn.1674-6090.2017.06.003
- VernacularTitle:肥胖大鼠袖状胃切除术后脂肪组织激素敏感性脂肪酶及肉碱脂酰转移酶-1表达变化
- Author:
Jian XU
1
;
Jiaobao HUANG
;
Ju WU
;
Min YIN
;
Nan CHENG
;
Jiajun YIN
Author Information
1. 大连大学附属中山医院肝胆腹腔镜外科
- Keywords:
Sleeve gastrectomy (SG);
Hormone sensitive lipase (HSL);
Carnitine acyl transferase-1 (CAT-1)
- From:
Chinese Journal of Endocrine Surgery
2017;11(6):451-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hormone sensitive lipase (HSL) and carnitine acyl transferase-1 (CAT-1) in Zucker rats after sleeve gastrectomy and to discuss the weight loss mechanism.Methods 30 male Zucker rats aging 10 weeks were randomly divided into 3 groups:the operation group (10 rats),the sham operation group (10 rats) and the diet-pairing group (10 rats).The rats were decapitated to retrieve the retroperitoneal adipose,mRNA and protein expression of HSL and CAT-1 gene of were detected by RTPCR and Western blot.Results As for the operation group,the weight decreased significantly after the operation comparing to the other two groups ((250±5.8) g,(370±10.0) g,(310±9.6) g,P＜0.05).mRNA and protein expressions of HSL and CAT-1 gene were all significantly higher in the operation group (P＜0.05).Conclusion SG can up-regulate the expressions of lipolysis gene HSL and CAT-1 in adipose in Zucker rats,promoting fat hydrolysis and increasing the energy expenditure,which is one of the important mechanisms of weight loss.
