Phenotypic Characteristics of CD8(bright) T Cells in Behcet's Uveitis.
- Author:
Jae Kyoun AHN
1
;
Hyeong Gon YU
;
Young Joo KIM
;
Young Suk YU
;
Hum CHUNG
Author Information
1. Department of ophthalmology, Seoul National University College of Medicine, Seoul, Korea. chungh@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Behcet's disease;
CD8(high)CD56+ T;
Cytotoxic T lymphocyte;
T-cell autoimmunity
- From:Journal of the Korean Ophthalmological Society
2005;46(7):1210-1216
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To determine the phenotypic characteristics of peripheral CD8(bright) T cells. METHODS: Forty patients with Behcet's disease (BD) with uveitis (active: 20; inactive: 20) and 20 healthy controls were recruited in this study. Phenotypic analysis of fresh PBMCs was performed using anti-CD8 mAb and anti-CD56 mAb in conjunction with a three- or four-color immunofluorescence tests for the expression levels of the following molecules: CD11b, CD27, CD45RA, CD45RO, CD62L, CD94, NKG2D, and HLA-DR. RESULTS: CD27 and CD62L were down-regulated on peripheral CD8(bright) T cells in patients with active Behcet's uveitis in contrast to the up-regulation of CD11b and HLA-DR. These phenotypic natures were remarkable in the CD56+ subsets. CD45RA(dim)CD45RO- phenotypes were expanded in peripheral CD8(bright)CD56+ T cells according to the disease activity. Interestingly, in active Behcet's uveitis, CD94 was up-regulated on peripheral CD8(bright)CD56+ T cells in BD in contrast to the down-regulation of NKG2D compared with the normal controls. CONCLUSIONS: CD8(bright)CD56+ T cells are characterized by activated effector phenotypes with functional NK receptors and they may play immunopathogenic roles in Behcet's uveitis.
