Effects of resveratrol on mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose conditions and its mechanism
10.3980/j.issn.1672-5123.2018.1.07
- VernacularTitle:白藜芦醇对高糖环境下视网膜血管内皮细胞线粒体DNA的影响及机制
- Author:
Yan WEI
1
;
Xiao-Qing SU
;
Hong LI
;
Bing WU
Author Information
1. 兰州总医院眼科中心
- Keywords:
resveratrol;
retinal vascular endothelial cells;
mitochondrial DNA copy;
mitochondrial transcription factor A;
proliferator-activated receptor coactivator-1 alpha
- From:
International Eye Science
2018;18(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate effects of resveratrol on mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose conditions and its mechanism.METHODS:Human retinal vascular endothelial cells were cultured in low glucose or high glucose medium,the genomic DNA was extracted and copy number of mitochondrial DNA was detected by real-time PCR.Effects of resveratrol on the mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose medium were studied.The expression of mitochondrial transcription factor A (TFAM) and acetylated proliferator-activated receptor coactivator-1 alpha (PGC-1α) were analyzed by Western blot and coimmunoprecipitation.RESULTS:High glucose inhibited the copy number of mitochondrial DNA in retinal vascular endothelial cells.However,resveratrol decreased the level of acetylated PGC-1α in retinal vascular endothelial cells,increased the expression of TFAM and the copy number of mitochondrial DNA.CONCLUSION:Resveratrol may improve mitochondrial function of retinal vascular endothelial cells exposed to a high glucose environment via activation of the PCG-1α-TFAM signaling pathway.
