Expression of FOXM1 in patients with esophageal squamous carcinoma and its clinical significance
10.13315/j.cnki.cjcep.2018.06.001
- VernacularTitle:食管鳞状细胞癌中FOXM1基因的表达及临床意义
- Author:
Pei-Qin LI
1
;
Shao-Fen YANG
;
Ya-Qian WENG
;
Chun-Hui ZHOU
;
Li-Na YU
Author Information
1. 广州卫生职业技术学院病理学教研室
- Keywords:
esophageal neoplasms;
squamous carcinoma;
FOXM1;
prognosis
- From:
Chinese Journal of Clinical and Experimental Pathology
2018;34(6):591-594,600
- CountryChina
- Language:Chinese
-
Abstract:
Purpose Analysis of correlation between FOXM1 gene expression levels and clinicopathological features and prognosis of patients with esophageal squamous cancer (ESC). The effect of down-regulation of FOXM1 expression on the proliferation of human ESC cell line KYSE-30 was also inves-tigated. Methods Quantitative real-time PCR (qRT-PCR) and immunohistochemistry ( IHC) methods were used to detect the expression of FOXM1 in ESC tissues and non-cancer tissues in mRNA and protein level. The expression of FOXM1 was down-regulated by RNA interference (RNAi) technique, and the pro-liferation activity of KYSE-30 cells was detected by CCK-8 as-say. Results Compared with the corresponding non-cancer tis-sues, the expression of FOXM1 was significant higher in ESC tis-sues(P＜0. 01). Meanwhile, the expression levels of FOXM1 in poorly differentiated esophageal carcinoma was higher than that in well-differentiated ESC group ( P ＜0. 01 ). The expression of FOXM1 was significantly correlated with poor tumor differentia-tion (P＜0. 001), lymphatic metastasis (P=0. 000), advanced stage (P=0. 004) of ESC patients after surgical resection. High FOXM1 expression was related to shorter overall survival ( OS) (P＜0. 001). After down-regulating FOXM1 expression in KYSE-30 cells, cell proliferation rate was inhibited (P＜0. 01). Conclusion FOXM1 expression is up-regulated in ESC and is closely related to the degree of differentiation, lymph node me-tastasis, clinical stage and prognosis of ESC. FOXM1 may be participated in regulating the proliferation of human esophageal carcinoma cell line KYSE-30.
