Characteristics of autophagy and its effect on cell proliferation in giant cell tumor of bone
10.13315/j.cnki.cjcep.2018.03.014
- VernacularTitle:骨巨细胞瘤细胞自噬特征及其对细胞增殖的影响
- Author:
Pan-Pan ZHANG
1
;
Rong-Zhu LU
;
Yan-Ming HAO
;
Ling-Na FANG
;
Da-Wei HE
;
Chong LI
Author Information
1. 江苏大学附属昆山医院检验科
- Keywords:
bone neoplasm;
autophagy;
giant cell tumor of bone;
cell proliferation
- From:
Chinese Journal of Clinical and Experimental Pathology
2018;34(3):305-309
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the autophagy characteristics of giant cell tumor of bone and its effect on cell proliferation. Methods Giant cell tumors of bone (GCT-0404 cells) were cultured in vitro and treated with serum-starvation, rapamycin and inhibitor chloroquine. Western blot analysis was performed to detect the autophagic markers LC3 and Beclin 1 expression. Immunofluorescence method was used to show intracellular autophagy formation. Inverted microscope was used to observe the cell morphology. CCK-8 assays were used to detect the cell viability. Results Green fluorescent spots that represented the transformation level of LC3-I to LC3-Ⅱ increased significantly (P＜0.05) by serum-starvation, rapamycin and inhibitor chloroquine respectively, but the expression level of Beclin 1 was not raised. In addition, the cell morphology changed significantly, and cell proliferation was inhibited after serum-starvation, and treatment with rapamycin and inhibitor chloroquine (P＜0.05), Conclusion The changes of autophagy caused by serum starvation, rapamycin and inhibitor chloroquine in GCT-0404 cells may be involved in the down-regulation of cell proliferation.
