Study of a novel diagnostic assay for thoracic malignant tumor based on DNA image cytometry

VernacularTitle:细胞DNA图像自动扫描与分析(DNA-ICM)技术在胸科恶性肿瘤诊断中的应用
Author: Jian-Ping XU ¹ ; Wei YE ; Jie-Ting ZHAO ; Rong-Rong SONG ; Kun NIE ; Li-Ping L(U)
Author Information

1. 安徽省胸科医院病理科
Keywords: thoracic tumor; DNA-ICM; ploidy analysis
From: Chinese Journal of Clinical and Experimental Pathology 2018;34(3):263-266
CountryChina
Language:Chinese
Abstract: Purpose To explore the effects of ploidy analysis on thoracic neoplasms based on DNA image cytometry (DNA-ICM), and to look for a meaningful novel diagnostic assay for tumor patients. Methods 4 402 patients who were diagnosed with thoracic disease were recruited in 2 years. By the DNA-ICM analysis, all the specimens were diagnosed as three types——positive, equivocal and negative ones. The results of701 specimens were compared with biopsy and clinical followup. Results DNA aneuploidy detected by DNA-ICM were65％ in confirmed malignant samples, 64％ in equivocal malignancy, and 8％ in non-malignant diseases. The comprehensive performance of DNA-ICM in malignancy was 73％, 93％, 71％, 94％ respectively for sensitivity, specificity, positive predictive value and negative predictive value. OR analysis found that the risk ratio of aneuploidy in malignancy was 23.236 compared to non-malignancy. Conclusion DNA-ICM can be applied in thoracic malignancy and have more potential values to be explored in oncology.