Endothelial cell protein C receptor promotes human breast cancer cell MCF-7 proliferation and migration by activating PAR-1
10.13315/j.cnki.cjcep.2018.03.002
- VernacularTitle:EPCR通过活化PAR-1促进人乳腺癌MCF-7细胞增殖及迁移
- Author:
Yang-Yang TANG
1
;
Yan-Yan XU
;
Hong-Li YANG
;
Qian ZHUO
;
Qing-Ling WANG
Author Information
1. 徐州医科大学病理学教研室
- Keywords:
breast neoplasm;
EPCR;
PAR-1;
proliferation;
migration
- From:
Chinese Journal of Clinical and Experimental Pathology
2018;34(3):244-247
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effect of EPCR on the proliferation and migration, and to explore the molecular mechanism of EPCR affecting the tumor growth and metastasis in human breast cancer cell line MCF-7. Methods MCF-7 cell was transfected with EPCR siRNA and treated with anti-PAR-1 antibody. Then CCK-8 assay was performed to determine the proliferation of MCF-7 cell. Transwell migration assay was employed to determine the cell's migration. Cell-ELISA was used to detect the activation of PAR-1 on the membranes of MCF-7. Result After EPCR siRNA transfection, the proliferation and migration ability of the MCF-7 in the interference of EPCR gene group was significantly decreased compared with the negative control and untreated control group. After treated with anti-PAR-1 antibody, the proliferation and migration of ability of MCF-7 were decreased significantly compared with the negative control group and the untreated control group. Cell-ELISA assay indicated that the activation of PAR-1 in the cells surface of MCF-7 cell in the EPCR gene interference group was mitigated versus the negative control and untreated control group. Conclusion EPCR may promote the proliferation and migration of MCF-7 cell by activating PAR-1.
